Report of Lawsonia intracellularis Infection in Dogs by Polymerase Chain Reaction
نویسندگان
چکیده
The purpose of this study was to survey Lawsonia intracellularis in dogs in Thailand by using Polymerase chain reaction technique. Lawsonia intracellularis is obligatory intracellular bacterium which causes proliferative enteropathy in animals, including dog. Fecal samples from 48 dogs were collected from Bangkok, Pathumthani and Nakhon Ratchasima and tested for L. intracellularis by PCR technique. The dogs were classified into two groups according to the presence or absence of diarrhea. In the group of dogs with diarrhea (n= 11), 6 dogs were positive (54.4%) and in the group without signs of diarrhea (n= 37), 15 dogs were positive (40.5%). The overall positivity was 43.8% (21/48). These results indicated that L. intracellularis was positive in dogs in Thailand.
منابع مشابه
Proliferative enteropathy involving Lawsonia intracellularis infection in rabbits (Oryctlagus cuniculus).
Five rabbits suffering from diarrhea were diagnosed with proliferative enteropathy (PE). Histopathology revealed a thickened mucosa consisting of hyperplastic intestinal epithelium and infiltration of inflammatory cells mainly consisted of macrophages. In the affected epithelial cytoplasm, numerous curved bacillus-like organisms were observed in the Warthin-Starry silver stain and electron micr...
متن کاملAssociation between faecal load of lawsonia intracellularis and pathological findings of proliferative enteropathy in pigs with diarrhoea
BACKGROUND The study was designed to investigate correlation between histological findings of Lawsonia intracellularis in porcine cases of diarrhoea and the quantitative detection of Lawsonia intracellularis in faeces. A total of 156 pigs (10 to 70 days post weaning) with diarrhoea were randomly selected from 20 herds: The pigs were subjected to necropsy, histopathology, immunohistochemistry an...
متن کاملFlotation—A New Method to Circumvent PCR Inhibitors in the Diagnosis of Lawsonia intracellularis
The obligate intracellular bacterium Lawsonia intracellularis causes enteritis and poor growth in weaned pigs. Cultivation is difficult and diagnosis ante mortem is mainly based on techniques such as polymerase chain reaction. However, false negative results caused by the presence of PCR-inhibitory factors constitute a problem. This study aimed to develop and evaluate a new technique, flotation...
متن کاملDevelopment of a polymerase chain reaction assay for quantification of Lawsonia intracellularis.
The objective of the present study was to develop a quantitative polymerase chain reaction (qPCR) assay using SYBR Green for quantification of Lawsonia intracellularis in cell culture and pig fecal samples. Specific primers were designed and tested using the aspartate ammonia-lyase (aspA) gene as a target. Serial 10-fold dilutions of cell culture samples and several sets of spiked feces were us...
متن کاملRapid Detection of Brachyspira Hyodysenteriae and Lawsonia Intracellularis in Swine Faecal and Mucosal Specimens by Multiplex Pcr
Multiplex polymerase chain reaction (M-PCR) was developed to determine its usefulness as a rapid and specific method for routine diagnosis of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in pigs. The assay was based on simultaneous amplification of the tlyA-encoded haemolysin gene of B. hyodysenteriae and 16S rRNA sequences of L. intracellularis. The results of this study ...
متن کامل